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SRX9084841: GSM4770472: WT 0D-Ctrl NPC; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 103.6M spots, 12.8G bases, 5.9Gb downloads

Submitted by: NCBI (GEO)
Study: Cell-Autonomous Requirement of Shp2 and ß-Catenin for Myc-Driven Hepatocarcinogenesis Revealed by Single Cell RNA-Sequencing
PRJNA661754 • SRP280277 • All experiments • All runs
show Abstracthide Abstract
We applied single-cell RNAseq analysis to characterize hepatic cell types, including hepatocytes (Hep) and non-parenchymal cells (NPC), in mouse liver, during Myc-induced hepatocellular carcinoma (HCC) development. Overall design: Hepatocytes (Hep) and Non-parenchymal cells (NPCs) were isolated from wild type (WT) and hepatocyte-specific Shp2 knockout (SKO) livers, at day 0 (0D-Ctrl) before HTVi, 10 days after Myc injection (10D-Myc), 10 days after empty vector injection (10D-Vector), and 4 weeks after Myc injection (4W-Myc).
Sample: WT 0D-Ctrl NPC
SAMN16070639 • SRS7331039 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Mouse liver was perfused with collagenase H (Roche). Hepatocytes and non-parenchymal cells were separated by centrifugation. Chromium Single Cell 3' Reagent Kits (V2 chemistry, 10x Genomics)
Experiment attributes:
GEO Accession: GSM4770472
Links:
Runs: 1 run, 103.6M spots, 12.8G bases, 5.9Gb
Run# of Spots# of BasesSizePublished
SRR12600856103,585,47612.8G5.9Gb2021-12-03

ID:
11804346

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